Application

Research CategoryEpigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected BAF180 in Western Blotting applications (Gao, W., et. al. (2017). Proc Natl Acad Sci U S A. 114(5):1027-1032).

Anti-BAF180, clone 370.1.8C3, Cat. No. MABE1138, is a highly specific rat monoclonal antibody that detects Protein Polybromo-1 (BAF180) and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein polybromo-1 (UniProt: Q86U86; also known as hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D) is encoded by the PBRM1 (also known as BAF180, PB1) gene (Gene ID: 55193) in human. BAF180 is a large, chromatin-remodeling protein that contains six bromodomains, two BAH-domains, and one HMG-box. Bromodomains recognize and bind to acetylated lysines, the BAH (bromo-adjacent homology) domains mediate protein protein interactions, and the HMG-box domain binds to DNA. Nine isoforms of BAF180 have been described that are produced by alternative splicing. BAF180 serves as a subunit of the SWI/SNF complex and plays critical roles in the regulation of chromatin remodeling and gene transcription, and is reported to be frequently mutated in several human cancers. BAF180 acts asa negative regulator of cell proliferation and plays a role in suppression of tumorigenesis through its ability to regulate both baseline and signal-dependent p21 transcription by its binding of the p21 promoter. Mutations in PBRM1 gene are known to cause renal cell carcinoma. (Ref.: Lee, H., et al. (2016). Oncotarget 7(15);19134-46; Gao, W., et al. (2017). Proc. Natl. Acad. Sci. USA. 114(5):1027-1032).

Immunogen

GST-tagged recombinant fragment corresponding to 241 amino acids from the C-terminal half of human Protein polybromo-1 (BAF180).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BAF180 in HEK293 cell lysate.

Specificity

Clone 370.1.8C3 is a rat monoclonal antibody that specifically detects human Protein Polybromo-1 (BAF180). It targets an epitope within 241 amino acids from the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~210 kDa observed; 192.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).